Identification of Prednisolone Acetate

Identification of Prednisolone Acetate IDENTIFICATION AND CHARACTERIZATION OF DRUG The procured prednisolone acetate was identified and characterized based on the following parameters. ORGANOLEPTIC PROPERTIES For the selected samples (drug + excipients) the following organoleptic properties were studied using descriptive terminology. Those were nature, odour and colour PARTITIONING BEHAVIOR OF PA IN DISTINCT LIPIDS: The percent partition coefficient of Prednisolone acetate in selected lipids was analyzed as elucidated elsewhere. On the part of the study/briefly, a mixture composed of melted lipid and 10mL of de-ionized water to this 10mg of PA was added. The resultant mixture was agitated reciprocally for one hour maintained at 50C above the lipid melting point. The quantity of lipid utilized was mentioned in table 1. The resultant dispersion was allowed to cool. Upon making cool both phases were separated by ultracentrifugation ( ) at 15,000 rpm and filtered. The drug content in supernatant was analyzed spectrophotometrically (240nm). The percent partition coefficient was predicted as follows: % partition = w1-w2/w1 x 100 W1 = Amount of drug added W2 = Amount of drug in aqueous phase DETERMINATION OF MELTING POINT PA melting point was determined by capillary method. Theoritical value was compared with practical value. LOSS ON DRYING This method measures the weight of volatile compounds or moisture of any kind which can be driven off under the specified conditions. Stoppered, shallow glass bottle was weighed and dried under the specified conditions. 2 grams of sample was accurately weighed and transferred to dried bottle. Then the loaded bottle was in electric oven, removed stopper and left near by it in drying chamber at 1050c for 2 hours at atmospheric pressure. After two hours the glass container was taken out from oven and weighed it again. The following formula was used for the calculation of LOD % LOD = Mass of test specimen – Mass of loaded bottle after drying/ Mass of sample – Mass of empty glass bottle IR SPECTROSCOPY OF DRUG Thin pellet was prepared using potassium bromide and drug in a ratio of 100:1 respectively. The molecular state of prednisolone acetate was studied using FTIR spectrometer. DSC PA thermogram was obtained in DSC 200 F3 Maia using empty aluminum pan as reference. The accurately weighed 2mg of sample was exposed to temperature range 0oc – 500oc under nitrogen atmosphere (flow rate: 60ml/min) at a heating rate of 10oc/min. UV-VISIBLE SPECTROPHOTOMETER (WAVE LENGTH SELECTION) 10mg of drug was dissolved in 100mL of simulated nasal electrolyte solution (SNES) which results in 100Â µg/ml concentration solution. From this 25 Â µg/ml was prepared. A few ml of this solution is transferred into cuvette (path length of 10mm cell) and scanned in the wave length range of 200-400nm against SNES as blank in double beam UV-Vis spectrophotometer. COMPATIBILITY STUDIES OF LIPID AND SURFACTANT MIXTURE The selected mixtures of lipid and surfactant, lipid and cosurfactant were blended at different ratios like 1:1, 2:1, 3:1 and 4:1. The blend was mixed for 10min and mixtures were observed visually for clarity (or) absence of turbidity. SOLUBILITY STUDIES OF PREDNISOLONE ACETATE The solubility of drug was estimated in different buffers, lipids, surfactant mixture ratios and solvents which are essential during development of formulation. IN DIFFERENT LIPIDS Test tube method was used for analysis of PA solubility in several solid lipids. To determine it 10mg of drug was placed to a test tube and temperature was maintained 50c higher/above the lipid melting point. The lipid was added an increase in quantity of 10mg till PA was solubilized completely and quantity of solid lipid needed for dissolution of drug was determined. IN VARIOUS RATIOS OF SURFACTANT MIXTURE The solubility of prednisolone acetate was investigated in numerous surfactant and co-surfactant ratios range from 1:1 to 4:1(Sur: Co-sur- Sur: Co-sur). Solubility studies were analyzed by adding an excess amount of PA in a 20 mL screw capped containers consisting different ratios of 10mL of each surfactant mixture. The mixtures were vortexed on orbital shaker at 50 rpm for 2 days at 37oc to enhance solubilization. After attaining equilibrium the samples were ultracentrifuged at 12,000 rpm for 30 min to separate the undissolved drug (PA) and supernatant was filtered through whatman filter paper. HPLC grade methanol was used to dilute the supernatant sample and these were filtered with 0.45 Â µm membrane filter. Then, the diluted samples were used to quantify the PA by UV-Vis spectrophotometer at 240nm. IN DIFFERENT SOLVENTS AND BUFFERS The excess quantity of drug was added to 10ml of different solvents includes pH 6, 6.4, 7, 7.4, 8 phosphate buffer, ethanol, double distilled water and methanol in a 25ml volumetric flask. Then flasks were properly capped and agitated at 37Â ±0.5oc in orbital shaker for 48 hours. The samples were filtered through whatman filter paper. The filtrate was diluted using suitable diluent and again filtered using 0.45Â µm membrane filters. Then samples were analyzed at 240nm by UV spectrophotometer. ds COMPATIBILITY STUDIES OF DRUG AND EXCIPIENTS The selected excipients were listed in GRAS (Generally recognized as safe by FDA) and used in various pharmaceutical formulations. Which are procured from reputed national and international manufacturers. The study of compatibility between drug and excipients was followed as. DSC METHOD : Differential scanning calorimetry was carried out using DSC F3 Maia instrument to analyze the melting transitions and heat capacity changes of drug alone and physical mixtures. The physical mixtures were prepared by triturating the PA and excipients in the ratio of 1:10 in a mortar for five minutes. The samples (5mg) were loaded in aluminum pans, sealed hermitically and heated under inert gas i.e. nitrogen at the flow rate of 60mL /min at 10oc/min heating rate ranges/set from 0 oc to 500 oc. The standard reference was an empty alumina crucible. The above said parameters/ conditions were used to analyze the samples and thermograms of DSC recorded/ data recorded using proteus software. Samples analyzed for DSC and FTIR were followed as The above physical mixtures compatibility was also found by adding desired quantity of mixture in glass vials and subjected to 60 oc for 30 days. The physical appearance of mixture was not changed at the end of the study. FTIR API was mixed with different excipients separately and spectral analysis was carried out using FTIR to study the placebo interference. The pressed pellet method was used to study the FTIR spectral studies. In this analysis, test-excipient mixture and potassium bromide were taken in the ratio of 1:100. The mixture when placed in hydraulic press under vacuum pressure of 800 mPa resulted in the formation of compressed thin transparent disc. The molecular states of samples were traced/determined between 4000-1000 cm-1 using Bruker FTIR spectrophotometer. The obtained IR spectra’s were evaluated to determine interactions. The data mentioned in table . Graphical representation shown/given in figure . METHOD DEVELOPMENT OF PREDNISOLONE ACETATE A survey of literature had showed that different instrumental methods like UV, HPLC, and HPTLC were reported for the quantification of prednisolone acetate. The present research work focused on development of UV spectroscopic method for dissolution samples estimation and HPLC for drug content, dissolution and bioanalytical quantification. Preparation of simulated nasal electrolyte solution(SNES) A solution of SNES was prepared by adding 8.77g of sodium chloride, 2.98g of potassium chloride, 0.45g of calcium chloride in water and finally made the volume to 100 mL resulting in pH 5.5. UV-Vis METHOD Stock solution prepartion 100mg of test sample was accurately weighed, transferred into 100 mL volumetric flask. To this little quantity of ethanol was added to dissolve the sample and made the volume up to 100ml using SNES (pH5.5) to get 1000Â µg/ml concentration stock solution. Prepartion of Linearity plot From the above stock solution 0.5, 1, 1.5, 2, 2.5ml were transferred in separate 100 mL volumetric flasks and diluted up to the mark with SNES to produce 5, 10,15, 20, 25 Â µg/ml concentrations respectively. The absorbance of each concentration was recorded in 1cm cell with SNES as blank at 240nm using Shimadzu, UV spectrophotometer. This procedure was done over for 3 times. With concentration on x-axis and absorbance on y-axis a calibration graph was constructed. Inter day and intraday studies were carried out for the determination of accuracy and precision using 1,5,10 Â µg/ml standard concentration solutions. The readings were tabulated in table and figure . HPLC METHOD PSEUDOTERNARY PHASE DIAGRAM CNSTRUCTION To construct the phase diagrams (prosim 1.0 software) hot water titration method was used. Through these phase diagrams components concentration range was investigated which can be results in existence of large microemulsion area. The surfactants used were tween 80,60,20, Cremophor RH40 and cosurfactants were ethanol, PEG 400. The surfactant and cosurfactants was blended in fixed weight ratios such as 1:1, 2:1, 3:1, 4:1. Solid lipids were tristearin, tripalmitin, GMS, stearic acid, Palmitic acid, cetyl palmitate. Lipid was melted at 65oc to this required quantity of hot surfactant mixture was added followed by adding water drop by drop to mixture under stirring with magnetic stirrer until turbidity formed. DEVELOPMENT OF DRUG LOADED MICROEMULSIONS From each phase diagram ME area was calculated by using CAD software. From each lipid, based on the above area values highest area of phase diagram was selected then the ME formulations were taken at required component weight ratios. The procedure of desired microemulsion was as follows. Required amount of lipid heated at 65oc in this 10mg of PA was dissolved. Heated SM and water was added to the above melted lipid mixture under stirring. The mixture was categorized as microemulsions if melt was appeared clear. Then mixture was considered as microemulsion. SOLID LIPID NANOPARTICLES PREPAR TION USING PROBE SONICATOR PA SLNs were prepared by hot lipid microemulsion technique. Drug loaded ME procedure was aforementioned in page No . 2mL of loaded ME was taken in a glass syringe using 22 gauge needle. Then it was added dropwise to a 3mL of cold de-ionized water under ice bath (2-4oc) and sonicated by probe sonicator for different time periods (5, 10, 15 min) specified by box behnken design at 200w amplitude to solidify the SLNs. The tip diameter of probe 8mm was dipped in liquid of 10mm leads to reflecting upwards and wave moving downwards. DRUG CONTENT (ASSAY) DETERMINATION 1 mL of solid dispersion from the formulations selected for evaluation was transferred into 10mL volumetric flask and diluted up to the mark with methanol. Then the solution was subjected to ultracentrifugation for 15 min at 10,000 rpm. Then the supernatant was aspirated, filtered, suitably diluted and measured the absorbance at ÃŽ »max 243nm. The results given in Table HR TEM The surface morphology of prepared SLNs like particle size, particle shape and aggregation were analyzed by HRTEM operating at 120kv voltage. The samples were diluted properly, stained by 2% phosphotungistic acid. A drop of aqueous dispersion was placed /mounted on carbon coated 400mesh copper grids which is to be allowed for 5 min to dry the nanoparticles on grid before examination under IR lamp/AIR DRY and TEM images were recorded with mangnification range from 11500-50000x. PARTICLE POTENTIAL, SIZE AND POLYDISPERSITY INDEX(PI) For the prepared SLNs the mean particle size, zeta potential and PI were performed by dynamic light scattering (DLS) with a zetasizer Nano – ZS90 (Malvern application center, banglore), model ZEN 3690 and equipped with 4mW, 633nm He-Ne laser. The measurements were made at the following conditions: Refractive index of medium: 1.330 Dielectric constant value: 78.5 Viscosity of dispersion medium: 0.8872 Temperature: 250c Then the samples (100Â µl) were diluted to 1mL with de-ionized water to prevent multi scattering and transferred in to a disposable zeta cuvettes to record the particle size. The size measurement angle was 900. The PI value was used to determine particle size distribution. The same instrument was used for the determination of zeta potential using an laser Doppler electrophoresis technique. Zeta potential value was calculated from the mean mobility of electrophoresis values by henry equation. The measurements were done in triplicate. ENTRAMENT EFFICIENCY % EE of the PA was studied by determining the unentrapped drug concentration in supernatant layer(aqueous layer). Accurately measured 5ml of (equivalent to 10mg of PA) nanoparticle dispersion was transferred to eppendorf centrifuge tubes and centrifuged at 15000 rpm for 30 min at -3oc using ultra cooling centrifuge. Then the supernatant was separated, diluted appropriately using SNES and filtered using 0.45 Â µm membrane filter. The filtered samples were estimated by using UV spectrophotometer at ÃŽ »max 240nm against blank. Encapsulation efficiency was determined using the equation. % EE = Quantity of drug added – weight of drug in aqueous phase/ mass of drug added x 100 INVITRO DIFFUSION STUDIES Dialysis bag method was used to determine invitro diffusion release profile of PA and using a USP dissolution apparatus II. Before using dialysis membrane was soaked in SNES for 12 hours and the molecular cutoff of membrane was between 12000-14000. The following conditions were used to carry out diffusion studies. The run speed of paddle: 100 rpm Diffusion medium: SNES (pH 5.5) Volume of medium: 500mL Temperature: 37Â ±0.5oc Time intervals: The SLNs dispersion containing the 10mg/5mL(amount equivalent to one dose of drug) was transferred in dialysis bag and tied at both ends. Then the it was suspended in the receptor compartment containing SNES pH 5.5. An aliquot of 5ml samples were removed at fixed time points from receptor medium. The same volume of fresh buffer was replaced after every time point to maintain constant buffer volume. The fresh buffer was also maintained at the same temperature(37Â ±0.5oc ) similar to sample. The PA concentration was estimated using UV-Visible spectrophotometer at 240nm against blank (SNES). The final optimized sample concentration was further determined by HPLC. The selected formulation results data were shown in Table and graphical representation in figure RELEASE KINETICS The release mechanism of drug from solid lipid nanoparticles were analyzed and determined by fitting the invitro release data to different kinetic models includes Zero, first order, higuchi and peppas-korsemayer. References UV-VISIBLE E.G.C. Clarke, Isolation and Identifeation of Drugs, volume 1 , The Pharmaceutical Press, London, 1978, p. 270

Students Need a Multicultural Classroom Essay -- Argumentative Persuas

Students Need a Multicultural Classroom I can identify with the multicultural concept of the classroom because I am African American, a woman, and a soon to be a teacher. This subject is one that is of extreme importance. The multicultural classroom is a learning environment that should be most effective because of the simple fact that it should encompass everyone. As a future teacher one of my goals for my classroom is to make it as diverse as possible. Not only because it should be a standard operating procedure, but because I feel students will be able to learn more effectively in an environment where they feel that their heritage is included. The definition of multiculturalism in Webster's Dictionary is "the existence, recognition or preservation of different cultures or cultural identities within a unified society." Therefore with this definition in mind a multicultural classroom is one that is the sum of all these things. A multicultural classroom is a learning environment that strives to reach all of its students, especially those of non-dominant ethnicities. In this environment students learn about the curriculum as well as their own cultural history. The aim of multicultural education is to ensure equity in education for all students and to help empower young people to make the world a better place both individually and collectively (Bigelow, 1993). Multicultural education is designed to help all students reach their full potential. Just about all teachers should have a desire to teach in a multicultural class because most classes in the United States of America contain at least one or two students of a differen t race or cultural background. This should not be considered a problem, but an asset because h... ...icultural Education: Development, Dimensions, and Challenges." Phi Delta Kappan. 75 (1), 22-28. Banks, J., and McGee, C. A. (Eds.). (1989). Multicultural Education: Issues and Perspectives. Boston: Allyn and Bacon. Bigelow, W. (1993). "Limits of the New Multiculturalism: The 'Good' Children's Literature and the Quincentenary." Unpublished manuscript. Available from NECA. Dietrich Fairtest. (1990). Standardized Tests and our Children: A Guide to Testing Reform. Massachusetts: National Center for Fair and Open Testing. Greenfield, E., and Little, L. J. (1979). Childtimes: A Three Generation Memoir. New York: Harper Collins. Hughes, L. (1960). The Dreamkeeper and Other Poems. New York: Alfred Knopf. Lee, E. (1993). "Strategies for Building a Multicultural, Anti-racist Curriculum." Presented at the Books Project Seminar, Washington, DC, May 8, 1993.

Ethnocentrism Tendency Essay

Ethnocentrism is the tendency to believe that one’s ethnic or cultural group is centrally important, and that all other groups are measured on one’s own cultural value. Ethnocentrism happens when one culture or nation places itself at the top of a self proclaimed hierarchy of cultures and nations and subsequently assigns other cultures and nations equivalent or lower value based on that scale. In other words, it is the proneness to think other cultures are of lesser worth because it is different from one’s own culture. Ethnocentrism helps to keep a culture from changing because it forces a culture to keep the same values and to be stubborn. If one culture bases other cultures off of their own hierarchy scale, then they are less likely to be open to accept changes that other cultures may have or want to bring to them because of the importance their own values and traditions. Cultural or ethnic groups develop traditions as a way to guide their population and to give guidelines in life. If one’s culture changes, then it may dismantle the entire society, causing confusion and chaos. For example, in the film, Dishonorable Killings: Punishing the Innocent, a man, who killed his own sister, explained that in his culture, family honor is extremely important because it represents the family as a whole in the community; it is more important than life. When a member, particularly a woman, dishonors the family, it shames the family and forces them to get rid the disgrace by killing her. Ethnocentrism can be both positive and negative for a cultural or ethnic group. It can be in a positive light because taking pride in beliefs and traditions elevates unity and solidarity among groups’ members, and it also strengthens the self-identity and self-esteem for individuals within those groups. It can also help societies establish and reinforce guidelines and expectations among coexisting people in all areas of the community. These expectations give societies the order and structure to hold them together, helping them run smoothly and allowing them to thrive. A negative view of ethnocentrism are the assumptions based on individuals’ own perception, that what works for them must work for others and that if it doesn’t, something is necessarily wrong, can cause harm, offense,  ill-feelings or otherwise put a damper on further relations between different societies. It could also cause oppression, prejudice, and cultural degradation, which can lead to actions ranging from conformity to violent protest.

Sixth Sense essays

Sixth Sense essays Many works of literature can be analyzed in many different ways. In the movie The Sixth Sense many aspects of the movie portray a great deal of religious symbolism. I will use the New Criticism approach to analyze these symbols. The uses of religious symbolism include Coles religious figurines taken from the church, the many trips and prayers in the chapel and the numerous prayers Coles says during the times he is frightened by the ghosts he sees. Coles religious figurines he has set up in his man-made red tent of Jesus, Virgin Mary and many angels is a way Cole tries to prevent the ghosts and demons from interfering with him. What is ironic about these figures is the fact that he stole them from a church, a religious building and betraying gods words of It is wrong to steal. Cole believes that these figures will allow god to protect him from these ghosts who he thinks are trying to kill him, but are really only trying to come to him for help as he thinks he is going to god for help with the figurines. Another ironic aspect to this is that Cole is afraid of the ghosts, but the only person he can talk to about his problems is the Doctor, who in turn is a ghost himself, who doesnt realize so. By keeping this distress to himself, Cole feels his life is uncontrollable and can not live a normal childhood like he would like. All of this is too much for a boy of young age to deal with, without the support of a older figure, bu t his mother still expects him to act like a normal child. Many times during the movie Cole visits the church sanctuary to pray to god for help. This location denotes good faith and godly duties, yet his youth is still plagued by the never-ending haunt of ghostly creature interfering with his conviction to god. The church denotes a false sense of a safe place in the movie. Cole often tells of the ghosts he sees, but i ...

Movie Fight Club essays

Movie Fight Club essays The movie, Fight Club, has many themes dealing with some of the class-discussed vocabulary. Through a scene by scene, and dialogue-based analysis of the movie, I have found that these themes are emphasized through discussions, interactions, and non-dialogue scenes between the main character, his imaginary sidekick and the society that has had such effect on the main character. Some of these themes or topics that are shared by both the movie and the class vocabulary appear randomly, sporadically, and repeatedly throughout the movie. Most of the scenes have mainly to do with the materialism in their society and its limits on the freedom, which the characters are trying to obtain. Others deal with how they, the movie's characters, feel a sense of alienation and this alienation distorts relationships developing due to their self-determination. There is also how family interactions help to shape our development on our vertical and horizontal relationships. Then finally, hedonism and how it affects the way we treat each other and how we interact within society. All the characters in the movie deal with and dissect these themes, in all that they say and how they react to the main characters disillusionment with his life; although the main characters are mostly the ones bringing the themes to the forefront of the movie. This any man, main character dislikes his life, even to the point that he is unable to sleep. He is disillusioned with his life, unhappy and does not understand why. And in order to feel anything he has to make a lot of bad choices to under go a life transformation. This transformation originates through his interactions and dealings with Tyler Durden, his alter ego and his imaginary friend. The main character remains without a name until in the end you, as the movie watcher, are lead to realize that he (the main chara...

Federal Express Secure Shipping Research Paper Example | Topics and Well Written Essays - 2500 words

Federal Express Secure Shipping - Research Paper Example Federal Express Custom Critical offers appropriate level of secure transportation of goods through secure standard operating procedures. Federal Express, FedEx Ground, FedEx Smart Post, FedEx Freight, FedEx Office, FedEx Custom Critical are the global operating units of Federal Express Corporation. The organization has effectively controlled the security issues by effective transportation and shipping strategy in international market. The customized secure services of Federal Express have provided its clients several flexible options and choices. Through secure packing and shipment, the organization has been able to monitor its shipments with much more detailing. With the help of covert devices the organization is successfully monitoring air and ground shipments. From premium airfreight to chartered aircraft, the organization has provided a solution to achieve transportation and monitoring needs. The study will reveal the shipping activities of Federal Express. Moreover, the study wi ll determine how the organization has improved shipping security for the purpose of easy and comfortable access. Securing Transport Solutions Available multi-sized charter aircraft in global market has helped the organization to bring pace in the organization’s goods delivery process in global market. Collaboration with the exclusive-use charter vehicle has helped the organization to ship goods and services in quick period of time. ... FedEx generally handles the air transportation process. For the purpose of additional security Federal Express has provided direct flight options at all transfer points in global market. Effective and in-depth communication activities with all FedEx locations, hubs and ramps have secured the international shipping services of goods (Gadman, Gadman & Cooper, 2009). Continuous shipment tracking in the air and on the ground have secured the transportation and shipping services. Sealed and dedicated container vehicle and blue urgent tape have helped the organization to identify the freights in order to ensure the security of shipping throughout the transit. FedEx Critical is the leader within the freight industry. The organization is popular for its exceptional client services, individual shipment customization and 24/7 availability of freight and goods pickup and delivery services. The organization used to pickup and deliver several differentiated products. Secured features within the B usiness Several leading companies around the globe are trying to capitalize on the potential opportunities that have been developed and created due to the globalization and modern advanced technology. Now-a-days, the service industry is achieving significant growth as the taste and preference of modern age’s customer has changed dramatically. However, transport security of global client’s critical shipment is becoming more important. The organization has provided an array of effective secure features. Federal Express has an extensive range of exclusive delivery vehicles that helped the organization to provide door-to-door direct services throughout Canada and United States. Two-way proactive

Enterprise and business development Essay Example | Topics and Well Written Essays - 500 words

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